Kevin Keomanee-Dizon

Founder, Coastal Applied Physics & Engineering (CAPE)

Dicke Fellow in Experimental Physics, Princeton University
Fellow, Center for the Physics of Biological Function
Ph.D., University of Southern California

Email  |  Scholar  |  CAPE  |  LinkedIn

I'm an experimental physicist, with a background in optics and a passion for invention. At USC and Princeton, and in industry, I've built microscope technologies to explore how structure and function emerge in complex living systems, from the dynamics of single molecules to computation in the brain.

We combine high-numerical-aperture single-objective detection with multiphoton plane illumination to resolve subcellular and single-molecule dynamics deep in multicellular systems through standard coverglass.

By applying two-photon light-sheet microscopy with visual stimuli to larval zebrafish, we map whole-brain activity at cellular resolution and trace the early emergence of "number neurons," revealing that the dynamics of neural networks can encode computation and abstract cognition in the brain.

We use a pupil phase mask to extend the detection depth of field to match the light-sheet thickness, turning out-of-focus fluorescence into useful signal and improving signal-to-noise and volumetric coverage.

We develop a Fourier transform-based method for snapshot hyperspectral imaging, enabling fast, photon-efficient fluorescence multiplexing, even at low signal-to-noise.

We adapt selective-volume illumination microscopy (SVIM) to a single-objective geometry, using an oblique one-photon illumination path or two-photon excitation to enable high-contrast light-field imaging.

This technology has been licensed to ZEISS.

We present an instrument with two independently controlled light-sheet microscopes sharing one multi-laser source, cutting system cost, and image a variety of specimens to demonstrate its versatility.

This technology is serving researchers at the USC Translational Imaging Center, and has also been adopted by research groups throughout the U.S.

We introduce SVIM, combining the high contrast of light-sheet microscopy with the synchronous volumetric acquisition of light-field microscopy, at rates limited only by the detector and available signal.

This technology has been licensed to ZEISS.

Good artists copy.